Starlight is a play loved throughout the world. Karen and Hikari make a promise with each other when they're young that one day they'll stand on that stage together. Time passes, and now the girls are 16 years old. Karen is very enthusiastic about the lessons she takes every day, holding her promise close to her heart. Hikari has transferred schools and is now away from Karen. But the cogs of fate turn, and the two are destined to meet again. The girls and other Stage Girls will compete in a mysterious audition process to gain acceptance into the revue.

In a world bursting at the seams with moe monsters and humanoids of the horned sort, which brave heroes will take it upon themselves to review the beastly babes of the red-light district? Can only one be crowned the ultimate title of best girl? Behold the most tantalizing of trials.

My childhood friend Shida Kuroha seems to have feelings for me. She lives next door, and is small and cute. With an outgoing character, she's the caring Onee-san type, this being one of her greatest strengths. ...But, I already have my first love, the beautiful idol of our school, and the award-winning author, Kachi Shirokusa! Thinking about it rationally, I should have no chances with her, but, while walking home from school, she only talks to me, with a smile even! I might actually have a chance, don't you think?! Or so I thought, but then I heard that Shirokusa already has a boyfriend, and my life took a turn for the worse. I want to die. Why is it not me?! Even though she was my first love... As I was drowning in despair and depression, Kuroha whispered. —If it's that tough for you, then how about we get revenge? The best revenge ever, that is~

Yayoi Sakamoto, a diehard fan of comedians and comedy acts, enrolls in the private Kazuki High School in Nanba (Osaka's entertainment district famous as the starting point for many comedians). She reunites with Yomogi Takahashi, a childhood friend who once formed the comedy duo "Konamonzu" with her when they were little. Before long, they find themselves putting together a routine at a park like they did before, in order to enter a local shopping area's contest. At that moment, a mysterious girl calls out to them.

New anime in the Revue Starlight franchise featuring chibi versions of the characters and the girls from the other schools from the mobile phone game.